<b>SIMPLE PURIFICATION OF RAT URINARY KALLIKREIN WITH AN IMMUNOAFFINITY </b><b>COLUMN </b>
نویسندگان
چکیده
منابع مشابه
Phytochrome immunoaffinity purification.
We have developed a phytochrome immunoaffinity purification procedure that yields undegraded oat (Avena sativa L., cv. Garry) phytochrome of greater than 98% purity within 2 hours when starting with a brushite-purified preparation. Immunoaffinity-purified phytochrome, except for its greater purity, is indistinguishable from conventionally purified phytochrome by gel exclusion chromatography, is...
متن کاملdevelopment of an immunoaffinity method for purification of streptokinase
background: streptokinase is a potent activator of plasminogen to plasmin, the enzyme that can solubilize the fibrin network in blood clots. streptokinase is currently used in clinical medicine as a thrombolytic agent. it is naturally secreted by β-hemolytic streptococci. methods: to reach an efficient method of purification, an immunoaffinity chromatography method was developed that could puri...
متن کاملIdentification, purification, and localization of tissue kallikrein in rat heart.
A tissue kallikrein has been isolated from rat heart extracts by DEAE-Sepharose and aprotinin-affinity column chromatography. The purified cardiac enzyme has both N-tosyl-L-arginine methyl ester esterolytic and kinin-releasing activities, and displays parallelism with standard curves in a kallikrein radioimmunoassay, indicating it to have immunological identity with tissue kallikrein. The enzym...
متن کاملDevelopment of an Immunoaffinity Method for Purification of Streptokinase
BACKGROUND Streptokinase is a potent activator of plasminogen to plasmin, the enzyme that can solubilize the fibrin network in blood clots. Streptokinase is currently used in clinical medicine as a thrombolytic agent. It is naturally secreted by β-hemolytic streptococci. METHODS To reach an efficient method of purification, an immunoaffinity chromatography method was developed that could puri...
متن کاملThe mechanism of urinary kallikrein excretion in the rat.
1. In male Wistar rats urinary kallikrein excretion was positively correlated with urinary flow and glomerular filtration rate (GFR). 2. Osmotic diuresis produced by a 30% (w/v) glucose solution increased urinary kallikrein, and a positive correlation between this variable and urine flow was observed. No correlation was observed with GFR. 3. The mechanism of urinary kallikrein excretion is inte...
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ژورنال
عنوان ژورنال: Biomedical Research
سال: 1982
ISSN: 0388-6107,1880-313X
DOI: 10.2220/biomedres.3.270